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massarray assay design genotyping software and tools  (Sequenom)

 
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    Sequenom massarray assay design genotyping software and tools
    Massarray Assay Design Genotyping Software And Tools, supplied by Sequenom, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/massarray+assay+design+genotyping+software+and+tools/massarray+assay+design+genotyping+software+and+tools/pmc08787081-84-17-18
    Average 90 stars, based on 1 article reviews
    massarray assay design genotyping software and tools - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: NR3C2 Gene is Associated with Susceptibility to High-Altitude Pulmonary Edema in Han Chinese.
    Article Snippet: Genomic DNA was extracted from venous blood by Gentra Puregene Blood Kit (Qiagen, 158389, Hilden, Germany) according to standard procedures. rs2070951 and rs5522 were genotyped by the single base extension detecting technology (iPLEX) (Capital Bio Corporation, Beijing, China). .. SNP loci tested polymerase chain reaction (PCR) primers and single-base extension primers were designed by using the Sequenom MassARRAY Assay Design Genotyping Software and Tools (Sequenom, San Diego, CA). .. The PCR reaction consisted of 4.0 mL of PCR Master Mixture (Takara Bio Inc, Kusatsu, Japan), 1 mL of primers mixture, 1.0 mL of genomic DNA (gDNA), 1.9 mL of ddH2O, and 0.1 mL of deoxynucleotide (dNTPs).

    Article Title: NR3C1 gene polymorphisms are associated with high-altitude pulmonary edema in Han Chinese
    Article Snippet: .. The primers for PCR and single-base extension were designed by using the Sequenom MassARRAY Assay Design Genotyping Software and Tools (Sequenom, San Diego, CA, USA). .. PCR was performed under the following thermal cycling conditions: 94 °C for 4 min, then 94 °C for 20 s, 56 °C for 30 s, and 72 °C for 1 min for 45 cycles, and 72 °C for 4 min. PCR products were treated with shrimp alkaline phosphatase to remove free deoxyribonucleoside triphosphates, and single-base extension reaction was performed in a system consisted of 2.0 mL of EXTEND MIX, 0.619 mL of ddH 2 O, 0.94 mL of Extend primer mix, 0.2 mL of iPLEX buffer plus, 0.2 mL of iPLEX terminator, and 0.041 mL of iPLEX enzyme (Sequenom, San Diego, CA, USA).

    Article Title: CYP17A1 and CYP2E1 variants associated with high altitude polycythemia in Tibetans at the Qinghai-Tibetan Plateau.
    Article Snippet: 24 25 26 27 28 29 30 31 32 33 34 Article history: Received 11 November 2014 Received in revised form 13 April 2015 Accepted 21 April 2015 Available online xxxx

    Article Title: EPAS1 Gene Polymorphisms Are Associated With High Altitude Polycythemia in Tibetans at the Qinghai-Tibetan Plateau.
    Article Snippet: All selected SNPs were genotyped by the Sequenom MassARRAY SNP assay (Capital Bio Corporation, Beijing, China). .. SNP loci–tested polymerase chain reaction (PCR) primers and single base extension primers were designed by using the Sequenom MassARRAY Assay Design Genotyping Software and Tools (Sequenom, San Diego, CA, USA). ..

    Article Title: Association Between the Polymorphism of Steroid Hormone Metabolism Genes and High-Altitude Pulmonary Edema in the Chinese Han Population
    Article Snippet: .. The primers for PCR and single-base extension were designed using Sequenom MassARRAY Assay Design Genotyping Software and Tools (Sequenom, San Diego, CA, USA). .. PCR was conducted under the following thermal cycling conditions: 94°C for 4 min, followed by 94°C for 20 sec, 56°C for 30 sec, and 72°C for 1 min for 45 cycles, then 72°C for 4 min. PCR products were treated with shrimp alkaline phosphatase to remove free deoxyribonucleoside triphosphates, and the single-base extension reaction was performed in a system containing 2.0 mL of EXTEND MIX, 0.619 mL of ddH2O, 0.94 mL of Extend primer mix, 0.2 mL of iPLEX buffer plus, 0.2 mL of iPLEX terminator, and 0.041 mL of iPLEX enzyme (Sequenom, San Diego, CA, USA).

    Software:

    Article Title: NR3C2 Gene is Associated with Susceptibility to High-Altitude Pulmonary Edema in Han Chinese.
    Article Snippet: Genomic DNA was extracted from venous blood by Gentra Puregene Blood Kit (Qiagen, 158389, Hilden, Germany) according to standard procedures. rs2070951 and rs5522 were genotyped by the single base extension detecting technology (iPLEX) (Capital Bio Corporation, Beijing, China). .. SNP loci tested polymerase chain reaction (PCR) primers and single-base extension primers were designed by using the Sequenom MassARRAY Assay Design Genotyping Software and Tools (Sequenom, San Diego, CA). .. The PCR reaction consisted of 4.0 mL of PCR Master Mixture (Takara Bio Inc, Kusatsu, Japan), 1 mL of primers mixture, 1.0 mL of genomic DNA (gDNA), 1.9 mL of ddH2O, and 0.1 mL of deoxynucleotide (dNTPs).

    Article Title: NR3C1 gene polymorphisms are associated with high-altitude pulmonary edema in Han Chinese
    Article Snippet: .. The primers for PCR and single-base extension were designed by using the Sequenom MassARRAY Assay Design Genotyping Software and Tools (Sequenom, San Diego, CA, USA). .. PCR was performed under the following thermal cycling conditions: 94 °C for 4 min, then 94 °C for 20 s, 56 °C for 30 s, and 72 °C for 1 min for 45 cycles, and 72 °C for 4 min. PCR products were treated with shrimp alkaline phosphatase to remove free deoxyribonucleoside triphosphates, and single-base extension reaction was performed in a system consisted of 2.0 mL of EXTEND MIX, 0.619 mL of ddH 2 O, 0.94 mL of Extend primer mix, 0.2 mL of iPLEX buffer plus, 0.2 mL of iPLEX terminator, and 0.041 mL of iPLEX enzyme (Sequenom, San Diego, CA, USA).

    Article Title: CYP17A1 and CYP2E1 variants associated with high altitude polycythemia in Tibetans at the Qinghai-Tibetan Plateau.
    Article Snippet: 24 25 26 27 28 29 30 31 32 33 34 Article history: Received 11 November 2014 Received in revised form 13 April 2015 Accepted 21 April 2015 Available online xxxx

    Article Title: EPAS1 Gene Polymorphisms Are Associated With High Altitude Polycythemia in Tibetans at the Qinghai-Tibetan Plateau.
    Article Snippet: All selected SNPs were genotyped by the Sequenom MassARRAY SNP assay (Capital Bio Corporation, Beijing, China). .. SNP loci–tested polymerase chain reaction (PCR) primers and single base extension primers were designed by using the Sequenom MassARRAY Assay Design Genotyping Software and Tools (Sequenom, San Diego, CA, USA). ..

    Article Title: Association Between the Polymorphism of Steroid Hormone Metabolism Genes and High-Altitude Pulmonary Edema in the Chinese Han Population
    Article Snippet: .. The primers for PCR and single-base extension were designed using Sequenom MassARRAY Assay Design Genotyping Software and Tools (Sequenom, San Diego, CA, USA). .. PCR was conducted under the following thermal cycling conditions: 94°C for 4 min, followed by 94°C for 20 sec, 56°C for 30 sec, and 72°C for 1 min for 45 cycles, then 72°C for 4 min. PCR products were treated with shrimp alkaline phosphatase to remove free deoxyribonucleoside triphosphates, and the single-base extension reaction was performed in a system containing 2.0 mL of EXTEND MIX, 0.619 mL of ddH2O, 0.94 mL of Extend primer mix, 0.2 mL of iPLEX buffer plus, 0.2 mL of iPLEX terminator, and 0.041 mL of iPLEX enzyme (Sequenom, San Diego, CA, USA).

    Amplification:

    Article Title: CYP17A1 and CYP2E1 variants associated with high altitude polycythemia in Tibetans at the Qinghai-Tibetan Plateau.
    Article Snippet: 24 25 26 27 28 29 30 31 32 33 34 Article history: Received 11 November 2014 Received in revised form 13 April 2015 Accepted 21 April 2015 Available online xxxx

    Synthesized:

    Article Title: CYP17A1 and CYP2E1 variants associated with high altitude polycythemia in Tibetans at the Qinghai-Tibetan Plateau.
    Article Snippet: 24 25 26 27 28 29 30 31 32 33 34 Article history: Received 11 November 2014 Received in revised form 13 April 2015 Accepted 21 April 2015 Available online xxxx



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